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KMID : 0870419980020010079
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Korean Journal of Hepato-Biliary-Pancreatic Surgery
1998 Volume.2 No. 1 p.79 ~ p.83
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Aflatoxin Related p53 Codon 249 Point Mutation in Korean Hepatocellular Carcinoma
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Wang Hee-Jung
Hong Seong-Woo
Park Young-Seok
Kim Wook-Whan
Kim Myung-Wook
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Abstract
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Purpose: P53 point mutation, especially G to T transversion at codon 249, has been found in many cases of the aflatoxin-related hepatocellular carcinoma(HCC). This study was aimed to exam the mutations of the third base at codon 249 of the p53 gene in Korean HCC.
Materials and Methods:Forty two patients with resectable HCC were enrolled. Genomic DNA was prepared by proteinase K digestion and phenol/chloroform extractions. A part of DNA including axon 7 of p53 was amplified by PCR. Restriction fragment polymorphism (RFLP) was analyzed after treatment of Hae III restriction enzyme. And the point mutation were confirmed using the direct sequencing method.
Results: P53 point mutation, G to T transversion at codon 249 was observed in only one tissue specimen(2.4%).
Conclusion: P53 point mutation at codon 249 does not correlate with HCC in Korea.
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KEYWORD
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Hepatocellular carcinoma, p53, codon 249, Aflatoxin
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